Cells were labeled with fluorescently tagged wheat germ agglutinin (WGA), which binds the bacterial cell envelope; multiple complementary gating strategies were employed for robustness. Here, we sought to (i) simultaneously assess the presence of RNAs produced from the proximal (early induced transcripts [early Q]) and distal (late Q) portions of the operon in individual cells, … Continue reading Cells were labeled with fluorescently tagged wheat germ agglutinin (WGA), which binds the bacterial cell envelope; multiple complementary gating strategies were employed for robustness